Purification of Substances from Achyrocline satureioides with Inhibitory Activity Against Paenibacillus larvae, the Causal Agent of American Foulbrood in Honeybees? Larvae

MARÍA J. GONZÁLEZ, ; VIVIANA G. BEOLETTO,; ALICIA M. AGNESE,; MARCELA C. AUDISIO; JUAN M. MARIOLI

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

HUMANA PRESS INC

Lugar: Oregon; Año: 2015 vol. 175 p. 3349 - 3349

0273-2289

bstract Achyrocline satureioides extracts were tested in vitro against the growth of Paenibacillus larvae. Four different extracts were obtained by liquid-liquid extraction from an aqueous-ethyl alcohol macerate of the aerial parts of the plant. The biological activity was tested by the broth microdilution technique. Hexane extract showed the highest activity (minimum inhibitory concentration=0.060±0.037 mg/mL). Transmission electron microscopy experiments showed that the main effect exerted by the hexane extract on the cell was at the cellular membrane level. The hexane extract was analyzed by thin-layer chromatography, and the activity of its components was tested by bioautography. Four growth inhibition zones were observed in the bioautographic experiments (using hexane-acetone (7:3) as mobile phase), with that at Rf=0.57 showing the largest zone of inhibition. High-performance liquid chromatographic experiments, using ultraviolet and electrospray ionization coupled to ta