1. Amino acids content and acetylcholinesterase inhibition of Huperzia saururus infusion and decoction.

VALLEJO M, ; DIMMER J,; ORTEGA M,; CABRERA J,; AGNESE M.

TAYLOR & FRANCIS INC

Lugar: Londres; Año: 2013 vol. 51 p. 1341 - 1341

ontext: Huperzia saururus (Lam.) Trevis. (Lycopodiaceae), an autochthonous plant species in Argentina, is used as a memory improver in traditional medicine. It was studied for this reason and the purified alkaloid extract did show significant activity on learning and memory. The species is mostly consumed in the form of infusions and decoctions. Objectives: To evaluate the activity of the H. saururus infusion and decoction as inhibitors of acetylcholinesterase (AChE) and to determine the amino acid content in both extracts. Material and methods: Infusion and decoction were purified by ionic exchange chromatography and analyzed by high-performance liquid chromatography HPLC-UV, and the AChE inhibition of these extracts was evaluated by using the Ellman method. Results: Both infusion and decoction exerted strong AChE inhibitory activities (IC50¼7.20.4 and 22.75.6 mg/mL, respectively). Among nine amino acids, arginine (Arg) was identified in a concentration greater than 9 mg/1