Antithrombotic ?in vivo? effects of quercetin 3,7,3?,4?-tetrasulfate isolated from Flaveria bidentis in an experimental thrombosis model in mice

AGNESE, ALICIA MARIEL

THROMBOSIS RESEARCH

PERGAMON-ELSEVIER SCIENCE LTD

Lugar: Amsterdam; Año: 2020 vol. 195 p. 190 - 190

0049-3848

he flavonoid quercetin tetrasulfate (QTS), a natural product isolated from Flaveria bidentis (L.) Kuntze, was previously shown "in vitro" to inhibit platelet aggregation and to possess significant anticoagulant and profibrinolytic effects. We designed the present study to determine the antithrombotic effects of QTS "in vivo", in an experimental model of thrombosis in male C57BL / 6 mice using an acute pulmonary thromboembolism test. Acute pulmonary embolism was induced by intraperitoneal (i.p) injection of a mixture of collagen and epinephrine after treatment with QTS at different concentrations. Paralyzed or dead mice in each group (controls and treatment) were counted and the percentages of protection against acute pulmonary embolism were calculated. In addition, tail bleeding tests, and blood loss, were determined in the presence and absence of QTS at 100 and 10 mg / kg, and compared with data from acetylsalicylic acid (ASA) treated mice. Histological examination of lung tissue sho