Institución organizadora:
Dpto de Farmacia Cs Químicas. UNC. Instituto Multidiciplinario de Biología Vegetal (IMBIV)
Resumen:
In previous reports we have demonstrated that the Huperzia saururus
(Lam.) Trevis. alkaloids have notable effects on CNS in related to
memory fenomena (1,2).
On the other hand, biodistribution of a drug
is an important parameter to be considered in early steps of a
pharmacological effects study in view that they can vary if the
concentration in the target organ is not the appropriated.
For this
reason, in the present work the concentration of sauroxina (Sx) (one of
the majority alkaloids) level is examined in rat brain.
With this
purpose, male Sprague-Dowley rats (60-70 days, 200-250 g) were injected
via i.p. with a dose of 30 mg/kg of chlorhidrate of Sx; 30 min later
rats were decapitated, their brains dissected and homogenized in HCl 0,1
M. After centrifugation, supernatant was alkalinized and partitioned
with ClCH3. This extract was analyzed by GLC-MS. For quantification, Sx
was used as external standard.
The obtained line equation allow us to
determine that the Sx concentration in the homogenate was of 0,33
mg/mL, so it can be deduced that the Sx content in the brain is 7,11
μg/g, representing a 0,13% of the initial administration.
These data
allow us to conclude that Sx cross the blood-brain barrier (BBB).
Besides, it is possible to asseverate that the percentage that cross the
BBB is very low, and that this would be enough to produce a
pharmacological effect.
According to previous experiments, were the
effect of Sx on learning and memory were studied in behavioral assays
(Step-down), 1 ng/rat was injected via intra-hippocampal showing an
important modification in these fenomena (2).
1) Vallejo M, et al., (2009) J. Nat. Prod.72, 156-8
2) Vallejo M (2009). Tesis doctoral. Dpto. de Farmacia, Fac. de Cs Químicas, UNC