Congresos y reuniones científicas
Título:
ALKALOID QUANTIFICATION IN A COMMERCIALIZED SAMPLE OF HUPERZIA SAURURUS (LYCOPODIACEAE)
Autor/es:
BIRRI M; CABRAL PÉREZ M; M VALLEJO; ORTEGA G,; CABRERA JL; AGNESE A
Reunión:
Simposio; X Simposio Argentino y XIII Latinoamericano de Farmacobotánica; 2010
Institución organizadora:
Dpto de Farmacia Cs Químicas. UNC. Instituto Multidiciplinario de Biología Vegetal (IMBIV)
Resumen:
Huperzia saururus (Lam.) Trevis., an Argentinean autochthonous species
is studied by our group because its effects on memory and learning.
Literature
refers about intoxications produced principally by decoctions consume.
Because of this, National Health Ministry published in 2002 a manual of
Primary Attention were associated risks of intoxication such as vomit,
diarrhea, convulsions and comma are described.
Statistical studies
previously done by us allowed us to demonstrate that 57.40% of surveyed
places in Cordoba city commercialized H. saururus. In relation to the
sale magnitude, 80.6% of the respondents said that comparatively it
represents 25% from the total of the most sold species. With respect to
the form of H. saururus commercialization, it is majority as a bulk or
fractionated, or in minor degree, under drops presentation.
Due to
the important consume of H. saururus it was objective of the present
work to quantify the present alkaloids in a commercial sample under a
drop presentation.
In order to obtain the alkaloids, the drops were
submitted to extraction with chloroform by means of a liquid-liquid
extractor with a previous media alkalinization until pH 12. Once the
chloroform extract was concentrated to dry, it was weighed and posterior
re-suspended in chloroform to give a final extract concentration of
22.2 mg/mL.
The separation and identification of the alkaloids were
achieved by GLC-MS. A calibration curve was prepared with quantification
purposes using ciclohexanone as internal standard and sauroxine (one of
the majority alkaloids) as analyte.
From the analysis of the
results, it can be concluded that the commercial sample has 0.01639
mg/mL of total alkaloids, expressed as sauroxine.
Since the
indication of usage is 30 drops three times a day, such concentration
implies a 0.025 mg of alkaloids consume per intake.