Dendronization of chitosan films: Surface characterization and biological activity

AGUSTINA ALDANA; BARRIOS B.; DARÍO ARRUA, DANIEL SERRANO, GUSTAVO PASTRANA, MIRIAM STRUMIA AND CECILIA ALVAREZ.; SILVIA CORREA; MARISA MARTINELLI

REACTIVE & FUNCTIONAL POLYMERS

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2016 p. 18 - 18

1381-5148

n advanced modified chitosan film was designed and one-side-dendronized chitosan was obtained (ChPW).Undendronized chitosan films ChP and ChPWwere characterized in terms of their chemical structure, morphology,and biological properties. Atomic force microscopy (AFM) and optical microscopy analysis were used tocorroborate the topological changes of the modified dendronized side of the ChPW film. Both macrophages(RAW) and fibroblasts (NIH) were cultured and observed on three different extracellular matrices: ChP, ChPW,and cell culture plastic (control groups). ChP and ChPWshowed noncytotoxicity toward RAW and NIH cells incontactwith these films. The increased arginase activitywith ChPWand ChP films suggests an alternative profileof L-argininemetabolismin RAW,which favors cell proliferation and wound healing. The results indicate that theChPW scaffold is favorable for wound dressing in skin tissue engineering.