Resumen:
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Redox and acid–base properties of thionine immobilized in crosslinked chitosan films are analyzed. The experimental conditions for the
preparation of the films are described. Results obtained with cyclic voltammetry, spectroelectrochemistry and spectrophotometry indicate that
the immobilized dye retains the redox and acid–base activity of free thionine. An acid dissociation constant (Ka) for the immobilized dye ofKa