Mucina/Carbopol matiz to immobilize oxalate oxidase in a urine oxalate amperometric biosensor

RAÚL CAPRA; MIRIAM STRUMIA; VADGAMA P.; ANA BARUZZI

ANALYTICA CHIMICA ACTA

Elsevier

Año: 2005 vol. 530 p. 49 - 49

0003-2670

p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; mso-layout-grid-align: none">An enzymatic amperometric electrode with extended analytical range and improved stability for oxalate determination has been developed. Glutarlaldehyde/mucin/carbopol matrix was used for the crosslinking of the enzyme between polymeric membranes to form a classical laminate construction (sandwich) and compared with the glutaraldehyde/mucin/enzyme and glutaraldehyde/albumin/enzyme. The use of a sulphonated membrane as internal membrane allowed rejection of the most important electrooxidable urine interferents. The recovery assays were highly satisfactory. The wide linear response in the range 2?400