Immunisation with a major Trypanosoma cruzi antigen promotes pro-imflammatory cytokines, nitric oxide production and increased TLR2 expression

GUIÑAZU N, PÊLLEGRINI A, CARRERA-SILVA EA, AOKI, MP, CABANILLAS A, GIRONES N, FRESNO M, CANO R, GEA S

INTERNATIONAL JOURNAL FOR PARASITOLOGY

ELSEVIER

Año: 2007 vol. 37 p. 1243 - 1243

0020-7519

nbsp; Abstract Innate and adaptive immunity collaborate in the protection of intracellular pathogens including Trypanosoma cruzi infection. However, the parasite molecules that regulate the host immune response have not been fully identified. We previously demonstrated that the immunisation of C57BL/6 mice with cruzipain, an immunogenic T. cruzi glycoprotein, induced a strong specific T-cell response. In this study, we demonstrated that active immunisation with cruzipain was able to stimulate nitric oxide (NO) production by splenocytes. Immune cells also showed increased inducible nitric oxide synthase protein and mRNA expression. Spleen adherent cells secret