Importance of TLR2 on Hepatic Immune and Non- Immune Cells to Attenuate the Strong Inflammatory Liver Response During Trypanosoma cruzi Acute Infection

EUGENIO ANTONIO CARRERA-SILVA,; NATALIA GUINAZU; ANDREA PELLEGRINI; ROXANA CAROLINA CANO ; ALFREDO AROCENA; MARIA PILAR AOKI ; SUSANA GEA

PUBLIC LIBRARY SCIENCE

Lugar: Genevieve Milon, Institut Pasteur, France; Año: 2010 vol. 4 p. 1 - 1

ackground: Toll-like receptors (TLR) and cytokines play a central role in the pathogen clearance as well as in pathological processes. Recently, we reported that TLR2, TLR4 and TLR9 are differentially modulated in injured livers from BALB/c and C57BL/6 (B6) mice during Trypanosoma cruzi infection. However, the molecular and cellular mechanisms involved in local immune response remain unclear. Methodology/Principal Findings: In this study, we demonstrate that hepatic leukocytes from infected B6 mice produced higher amounts of pro-inflammatory cytokines than BALB/c mice, whereas IL10 and TGFb were only released by hepatic leukocytes from BALB/c. Strikingly, a higher expression of TLR2 and TLR4 was observed in hepatocytes of infected BALB/c mice. However, in infected B6 mice, the strong pro-inflammatory response was associated with a high and sustained expression of TLR9 and iNOS in leukocytes and hepatic tissue respectively. Additionally, co-expression of gp91- and p47- phox NA