Response of triiodothyronine (T3)-dependent enzyme activities to insulin like growth factor I (IGF-1) and growth hormone (GH) in cultured rat hepatocytes

PELLIZAS CLAUDIA,; COLEONI ALDO,; ANA MARIA DE LOS A. CABANILLAS; MASINI ANA,; COSTAMAGNA MARIA,

EUROPEAN JOURNAL OF ENDOCRINOLOGY

European society of Endocrinology

Año: 1996 vol. 134 p. 215 - 215

0804-4643

p>Triiodothyronine (T3) is involved in the regulation of the growth hormone-insulin-like growth factor I (GH-IGF-I) axis. In this study we investigated the effect of GH and IGF-I on the metabolic response of T3 in target tissues by evaluating the activity of two T3-dependent liver enzymes: mitochondrial -glycerophosphate dehydrogenase (-GPD) and cytosolic malic enzyme (ME) in rat hepatocytes in primary culture. Growth hormone (35 nmol/l) as well as IGF-I (0.5 µmol/l) reduced -GPD and ME activities (p < 0.01) compared to the control group. Timecourse studies indicated that IGF-I 1.5 µmol/l) significantly decreased