Alternative splicing gives rise to two isoforms of Zfhep, a zinc finger/homeodomain protein that binds T3 response elements

ANA MARIA DE LOS A. CABANILLAS; DARLING DOUGLAS,

DNA AND CELL BIOLOGY

Mary Ann Liebert

Lugar: New Rochelle, NY, USA; Año: 1996 vol. 15 p. 643 - 643

1044-5498

p class="MsoNormal" style="MARGIN: 0cm 0cm 0pt; mso-layout-grid-align: none">We have previously isolated a cDNA for a transcription factor referred to as Zfhep (Zinc Finger Homeodomain Enhancer-binding Protein) containing two separate zinc finger domains, ZD1 and ZD2, each of which binds DNA, and a homeodomain. The rat Zfhep cDNA lacks a 5'-methionine codon, present in some homologs from other species. Hence, the aim of this work was to isolate the 5'-end of the rat Zfhep cDNA. Zfhep-2 cDNA was isolated, having a total length of 2.5 kbp, including more than 1.1 kbp of novel sequence followed by 1.4 kbp identical to the Zfhep-1 clone. The 1.1 kbp of novel sequence contains multiple stop codons in all reading frames, suggesting that it represents the 5'-untranslated (5'-UT) region of the rat Zfhep-2