Atrial natriuretic peptide inhibits iodide uptake and the thyroglobulin messenger ribonucleic acid expression in cultured bovine thyroid follicles

MARIA COSTAMAGNA,; ALDO COLEONI,; CLAUDIA PELLIZAS,; ANA MARIA DE LOS A. CABANILLAS; MARIA VELEZ,; ANA MASINI,

Elsevier

Lugar: Amsterdan; Año: 2002 vol. 106 p. 19 - 19

font size="1" face="AdvTT5843c571"> The involvement of atrial natriuretic peptide (ANP) in the regulation of thyroid gland is supported by the presence of high-affinity ANP receptors and the identification of the peptide in thyroid follicular cells. The aim of this work was to study the action of ANP on parameters of thyroid hormone biosynthesis and analyze the intracellular mechanism of the ANP action in cultured bovine thyroid follicles. The addition of ANP (0.1?10 nM) to the culture medium for 24 h inhibited the TSH (thyroid-stimulating hormone)-stimulated iodide uptake with a maximal inhibition at 1 nM ANP. When thyrocytes were incubated with 10 nM ANP the inhibitory effect slightly increased from 24 to 72 h. Thyroglobulin (Tg) mRNA expression was reduced by 1 and 10 nM ANP. After 24 h of treatment with the cGMP analogue,