Phosphorylation Regulates Functions of ZEB1 Transcriptional Factor

LLORENS DE LOS RIOS C; LORENZATTI, GUADALUPE; CAVALLO N; VAGLIENTI, M. VICTORIA; PERRONE A; CARENBAUER, A; DARLING, DS; CABANILLAS, AM

WILEY-LISS, DIV JOHN WILEY & SONS INC

Año: 2016

!--[if gte mso 9]> ZEB1 transcription factor is important in both development anddisease, including many TGFb-induced responses, and the epithelial-to-mesenchymal transition(EMT) by which many tumors undergo metastasis.  ZEB1 is differentially phosphorylated indifferent cell types; however the role of phosphorylation in ZEB1 activity isunknown.  Luciferase reporter studies andelectrophoresis mobility shift assays (EMSA) show that a decrease in phosphorylationof ZEB1 increases both DNA-binding and transcriptional repression of ZEB1target genes.  Functional analysis of ZEB1phosphorylation site mutants near the second zinc finger domain (termed ZD2)show that increas