HPLC method for the determination of nystatin in saliva for application in clinical studies

J.M. LLABOT, D.A. ALLEMANDI, R.H. MANZO, M.R. LONGHI

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

Elsevier Sciences

Lugar: Oxford, UK; Año: 2007 vol. 45 p. 526 - 526

0731-7085

p class="MsoNormal" style="TEXT-JUSTIFY: inter-ideograph; MARGIN: 0cm 0cm 0pt; LINE-HEIGHT: 200%; TEXT-ALIGN: justify">An isocratic high-performance liquid chromatographic method was developed, optimized and validated for the determination of Nystatin in human saliva (UV and Fluorescence detection).  A reversed-phase Luna C18 column, (25 ºC), with a mobile phase of  MeOH, H2O, and DMF (70:20:10 v/v/v), and a flow-rate of 0.8 mL/min were used. The elution time for Nystatin was 5.8 +/- 0.2 min. Calibration curves in human saliva were linear from 0.78 to 50 ug/mL. Limits of quantification were 0.78 ug/mL and 0.75 ug/mL for UV and Fluorescence detection, respectively. The accuracy and precision values of intra- and inter-day variation studies were within acceptable limits, according to FDA guidelines. The described method has probed to be