Cucurbit[6]uril nanocavity as enhanced spectrofluorimetric method for the determination of pyrene.

V. N. SUELDO OCCELLO ; A. V. VEGLIA

ANALYTICA CHIMICA ACTA

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2011 vol. 689 p. 97 - 97

0003-2670

he effect of the addition of macrocyclic host cucurbit[6]uril (CB6) on the photophysical properties of polycyclic aromatic hydrocarbon pyrene (PYR) was analyzed. The fluorescence emission spectra of the aromatic compound were determined at 25.0 ◦C in different acidic media (HCl 18%, w/v, or HCOOH 55%, w/v) with and without CB6. A significant enhancement in the fluorescence signals in the presence of CB6 was observed. The average values of the association constant (KA) for the 1:1 stoichiometry complex and the relative fluorescence quantum yield ratio between the complexed and free PYR (PYR?CB6/PYR) in acidic media were (4.0 ± 0.5) × 102 M−1 and (5.7 ± 0.2), respectively. The analytical parameters improved in the presence of CB6. The relative decrease in the limit of detection was 92%. The matrix effect was evaluated in fortified samples of tap water and tea extracts. Apparent recoveries obtained by the proposed method in tap water and tea extracts were (82?103)% and (89?99