Resumen:
nanosensor based on β-cyclodextrin (βCD) macrocycles linked to gold nanoparticles for rhodamine B (RhB)sensing was developed applying the metal-enhanced fluorescence effect (MEF). Hence, we have developed many ways to control the distance of supramolecular systems to nanoparticle surface with different bioconjugation strategies in order to optimize signal detection. Different PEG spacer arm lengths were used to cover the nanoparticle surface with molecular spacers. This type of molecular shell is biocompatible, enabling to switch on/off the MEF effect using a dithiane linker by a simple reduction reaction. In the presence of the nanosensor obtained, an increase was observed in RhB fluorescence emission depending on molecular length, that is a characteristic effect ofMEF. Themajor increasemeasuredwas 60% comparedwith RhB emission in buffer at 1 nMlevel, for a spacer length of 3.58 nm and an 80% increase as compared with that in the presence of βCD. These differences are