Spectrofluorimetric determination of Serotonin and 5-hydroxyindoleacetic acid in urine with different cyclodextrin media

A. GUILLERMO BRACAMONTE ; A. V. VEGLIA

TALANTA

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2011 vol. 83 p. 1006 - 1006

0039-9140

lternative and sensitive spectrofluorimetric methods for the determination of hydroxyindoles, such as serotonin (5HT) and 5-hydroxyindoleacetic acid (5HIA), were developed on the basis of supramolecular interaction with cyclodextrin (CD) nanocavities (CD and hydroxypropyl-CD, HPCD) at different pH values. Both substrates and receptors have acidic protons, therefore the interactions produced in different systems were considered. The effects of neutral CD at pH 2.00 and 6.994, and of anionic CD at pH 13.00 on the specific acid-base species of the compounds at each pH were determined. In all the conditions studied, the fluorescence of the substrates in the presence of CD increased. The association constants (KA, mol−1 L) between the substrates and CD were determined (30?300) and interpreted. A zero-crossing first-derivative spectrofluorimetric method with and without HPCD was developed for the simultaneous determination of 5HT and 5HIA. The limits of detection (LD, ng mL−1) f