In Flow Metal Enhanced Fluorescence for Biolabelling and Biodetection

GONTERO, DANIELA; VEGLIA, A. V.; BRACAMONTE, A. G.

Photochemical and Photobiological Sciences

ROYAL SOC CHEMISTRY

Lugar: CAMBRIDGE; Año: 2020 p. 1 - 1

1474-905X

scherichia coli bacteria were determined by in flow cytometry with laser excitation and fluorescencedetection applying ultraluminescent core?shell nanoparticles based on Metal Enhanced Fluorescence(MEF). Core?shell nanoparticles consisted of a 40 nm core modified with a silica spacer grafted withRhodamine B (RhB). The electromagnetic field in the near field of the core surface enhanced the fluorescenceof RhB by plasmonic and fluorophore coupling. The hydrophilic silica spacer allowed the noncovalentinteraction with the polar E. coli surface and thus ultraluminescent bacteria biolabelling wasdeveloped. Clearly, well defined and bright bacteria imaging was recorded by Laser FluorescenceMicroscopy based on the non-covalent deposition of the ultraluminescent nano-emitters. Using thesenano-labellers, it was possible to detect labelled E. coli by in flow cytometry. Higher values of Side-scatteredlight (SSC) and Forward-scattered light (FSC), and number of fluorescent event detections, wereo