Ultraluminescent gold core-shell nanoparticles applied to individual bacterial detection based on metal-enhanced fluorescence nanoimaging

VEGLIA, ALICIA V.

JOURNAL OF NANOPHOTONICS

SPIE-SOC PHOTOPTICAL INSTRUMENTATION ENGINEERS

Año: 2018 vol. 12 p. 1 - 1

1934-2608

bstract. Gold core?shell nanoparticles were synthesized based on metallic cores, variablesilica shell spacers covered with modified fluorescent silica layers. Ultraluminescent propertieswere obtained based on metal-enhanced fluorescence (MEF). Different silica spacers were synthesizedto optimize the MEF enhancement factor (MEFEF). An optimal MEFEF was determinedequal to 9.5 for shorter silica spacers (d−SiO2− ¼ 10 nm). These nanoparticles were deposed onEscherichia coli bacteria at different concentration levels for Bioimaging generation over theirsurfaces. The best luminescent nanoparticles were deposed on intermediate and higher bacteriaconcentrations. In the presence of intermediate bacteria concentrations, the ultraluminescentnanoparticles adsorbed showed an increase of 35% to 45% compared with individual nanoparticles.To modify the surface of individual bacteria, diluted samples of bacteria were used inwhich a 20% decrease in fluorescence emission was measured. In the