Inflow nano-optics from the near-to the far-field detection based on Metal-Enhanced Fluorescence signaling

GOMEZ PALACIOS, LUNA R.; VEGLIA, ALICIA; BRACAMONTE, A. GUILLERMO

MICROCHEMICAL JOURNAL

ELSEVIER SCIENCE BV

Año: 2021 vol. 169

0026-265X

n this communication we evaluated the detection and tracking of Ultraluminescent gold core?shell nanoplatformsbased on Metal-Enhanced Fluorescence (MEF) within colloidal dispersion by In-Flow Cytometry andImaging Flow Cytometry (IFC). The enhanced properties were developed by the incorporation of Rhodamine B(RhB) as a laser dye reporter at variable controlled silica spacer lengths (Au@SiO2-RhB) from a gold coretemplate. Thus, SiO2 was varied from 6 to 7 nm to 25 nm in order to place the laser dye sensitizer at differentlengths of the higher electromagnetic fields surrounding the 45-nm gold core diameter. By Laser FluorescenceMicroscopy, high intense emissions were shown at shorter SiO2 lengths, while lower emissions were found towardslonger ones. These characteristics related to MEF were evaluated by in-flow cytometry with dual laserexcitation and fluorescence detection, and by an IFC set-up. By In-flow cytometry, different scattered-lightdistributions were shown, depending on the nan