Self-assembly dynamics and effect on synthetic nanobio-optical properties by hybrid monocolored silica nanoparticle labeling of Escherichia coli

GOMEZ PALACIOS, LUNA R.; SALINAS, CARINA; VEGLIA, ALICIA V.; AMÉ, MARIA VALERIA; BRACAMONTE, ANGEL GUILLERMO

JOURNAL OF NANOPHOTONICS

SPIE-SOC PHOTOPTICAL INSTRUMENTATION ENGINEERS

Año: 2022 vol. 16

1934-2608

he deposition of silica nanoparticles on Escherichia coli bacteria was investigated. The noncovalent interaction between the silanized surfaces and polar components of the biomembrane resulted in a nanobiostructure. This hybrid architecture showed stable conformation characteristics evaluated with different microscopy techniques, such as bright field confocal microscopy and transmission electron microscopy (TEM). Nanobioarchitectures were detected within colloidal dispersions and in the absence of aqueous media. Nanobiointeractions were related to strong polar and hydrogen bridges´ noncovalent interactions. Thus, well-constituted and defined nanobiostructures were observed by bright field confocal microscopy and TEM after their preparation in optimal conditions. However, to evaluate their stability and internanobiostructure interactions, size distributions within variable periods were determined. Variable nanobioaggregate sizes were recorded according to nanoparticles and bacteria con