Tracking metal enhanced fluorescence from Eschericcia coli nano-bio-assemblies within colloidal dispersions by static and 3D spectra emissions

GOMEZ PALACIOS, LUNA R.; VEGLIA, ALICIA V.; VALERIA AMÉ, MARIA; BRACAMONTE, A.GUILLERMO

MICROCHEMICAL JOURNAL

ELSEVIER SCIENCE BV

Año: 2023 vol. 190

0026-265X

t was studied the interactions of single Ultraluminescent 40 nm gold Core shell Nanoparticles (d40 Au@SiO2-Fl) with Eschericcia Coli bacteria (Esc. Col). By this manner, it was tracked from initial Nano-Bio-assembly formation (Au@SiO2-Fl-Esc. Col) towards inter-Nano-Biostructure interactions. In order to do that it was determined association constants between d40 Au@SiO2-Fl and Biostructures by 3D Fluorescence, fluorescence spectroscopy measurements. The association constant was related with non-covalent Nano-Biostructure interactions of 45 ± 2 biostructure-1; accompanied with a Quantum Yield ratio between the Bio-assembly and Nano-labeller of 3.6 ± 0.1 times, with the maximal concentration of bacteria addition. This phenomenon of interaction was tracked from the bulk of the colloidal dispersion. And, from these individual interactions, it was possible to generate Bioimaging by Laser Fluorescence Microscopy in real time of the dynamic of Bio-assembly formation as well as the by inter-