c-Fos activated phospholipid synthesis is required for neurite elongation in differentiating PC12 cells

GIL GA,; BUSSOLINO DF,; PORTAL MM,; PECCHIO AA,; RENNER ML,; GRACIELA ADRIANA BORIOLI; GUIDO ME,; CAPUTTO BL,

MOLECULAR BIOLOGY OF THE CELL

Año: 2004 vol. 15 p. 1881 - 1881

1059-1524

We have previously shown that c-Fos activates phospholipid synthesis through a mechanism independent of its genomicAP-1 activity. Herein, using PC12 cells induced to differentiate by nerve growth factor, the genomic effect of c-Fos ininitiating neurite outgrowth is shown as distinct from its nongenomic effect of activating phospholipid synthesis andsustaining neurite elongation. Blocking c-Fos expression inhibited differentiation, phospholipid synthesis activation, andneuritogenesis. In cells primed to grow, blocking c-Fos expression determined neurite retraction. However, transfectedcells expressing c-Fos or c-Fos deletion mutants with capacity to activate phospholipid synthesis sustain neurite outgrowthand elongation in the absence of nerve growth factor. Results disclose a dual function of c-Fos: it first releases thegenomic program for differentiation and then associates to the endoplasmic reticulum and activates phospholipidsynthes