Autor/es:
GRACIELA ADRIANA BORIOLI; THOMPSON-COFFE, C; D. ZICKLER,; A. ROSA,
Resumen:
hompson-Coffe, C., Borioli, G., Zickler, D., and Rosa,A. L. 1999. Pyruvate decarboxylase filaments are associatedwith the cortical cytoskeleton of asci and sporesover the sexual cycle of filamentous ascomycetes.Fungal Genetics and Biology 26, 71?80.We show thatpyruvate decarboxylase (PDC) 8- to 10-nm-diameterfilaments, first described in vegetative cells of Neurosporacrassa, are ubiquitously present in filamentousfungi. The cellular arrangement of these structures wasexamined over the entire sexual cycle of the ascomycetesN. crassa, N. tetraesperma, Podospora anserina,and Sordaria macrospora. PDC-filaments were foundassociated with the cortical microtubule array of asciand ascospores and absent from the vicinity of spindlesand spindle pole bodies. Nocodazole-induced depolymerizationof the cortical microtubules results in theloss of PDC-filaments. Moreover, a S. macrosporamutant defect