c-Fos and phosphatidylinositol -4,5-biphosphate reciprocally reorganize in mixed monolayers.

GRACIELA ADRIANA BORIOLI; CAPUTTO B.L,; MAGGIO B.,

Science Direct

Año: 2005 vol. 1668 p. 41 - 41

he transcription factor c-Fos has surface thermodynamic properties that allow it to differentially interact with phospholipids, especiallyPIP2. It regulates phospholipid metabolism both in vivo and in vitro, and modulates degradation of phospholipid monolayers byphospholipases in a way that depends on the membrane intermolecular packing (i.e., surface lateral pressure). With the aim to understanddetails of the interactions of c-Fos at the membrane level, we studied the surface packing, dipole potential, compressibility and topography ofmixed films of the protein with PIP2. We show that c-Fos changes the packing of liquid-expanded PIP2 monolayers, in a different mannerwith respect to its effect on the similarly liquid-expanded dilauroylphosphatidylcholine monolayers. The changes at the local molecular levelare transduced to long-range inhomogeneities of the surface, detected by Brewster angle (BAM) and epifluorescence microscopy (EFM). Ourresul