EARLY INFLAMMATORY MARKERS IN ELICITATION OF ALLERGIC CONTACT DERMATITIS.

MARTÍN, AP; GALLINO, N; GAGLIARDI, J; ORTIZ, S; RUIZ LASCANO, A; DILLER, A; DARAIO, MC; KAHN, A; MARIANI, AL; SERRA, HORACIO MARCELO

Bmc Dermatology

BMC

Año: 2002 vol. 2 p. 1 - 1

1471-5945

ackground: Allergic Contact Dermatitis (ACD) is regarded as a T-cell-mediated delayed-typehypersensitivity reaction. We studied the kinetics of the expression of CS-1 fibronectin, thymusand activation-regulated chemokine (CCL17/ TARC) and different chemokine receptors (CR) inskin biopsies from individuals suffering from back problems, with the antigen responsible of theircontact dermatitis and an irrelevant antigen.Methods: Samples were taken at 2, 10, and 48 hours for histological and immunohistochemicalstudies using monoclonal antibodies against human CS-1 fibronectin, CCL17, CD3, CD68, CD49d,CXCR3, CCR5, and CCR3.Results: At positive antigen stimulated sites there was an early expression of CS-1 fibronectin (2hours), followed by CCL17 and a later accumulation of alplha4/beta1+ (CD49d), CD3+, CD68+,CXCR3+ and CCR5+ mononuclear cells. At 48 hours, approximately 59 % of infiltrating cells wereCXCR3+, 42% CCR5+, and only