STUDY OF N-LINKED GLYCOPROTEINS IN TEARS FROM PATIENTS WITH CLIMATIC DROPLET KERATOPATHY IN ARGENTINA

SERRA H M; R.W. BEUERMAN,; L. ZHOU,; AI PING CHEW,; SIEW KWAN KOH; CAFARO T A,; URRETS-ZAVALIA EA; URRETS ZAVALIA, JA; LI S

MIAMI

Congreso; ARVO 2009; 2009

Since glycoproteins are potentially biomarkers of diseases we decided to investigate N-linked glycoproteins in tears of CDK patients and compare these profiles with tears from normal individuals. Methods: 13 CDK patients and 11 control subjects with clinically normal eyes and no history of eye disease or eye surgery were recruited for this study.Tear samples were pre-fractionated using a hydrazide-resin capture method, and the previously N-glycosylated peptides were then subjected to two-dimensional nano-LC-nano-ESI-MS/MS analysis to obtain peptide fragmentation patterns for identification through protein database searches. Quantitative comparisons of N-glycoprotein profiles in tear fluid of patients with CDK and to that of non-diseased controls used glycopeptide capture, iTRAQ labeling and 2D nano-LC-nano-ESI-MS/MS analysis. The significant up-regulation of N-glycosylated haptoglobin in CDK, which is related to exposure to ultraviolet irradiation, could be clinically significant in terms of disease etiology and biomarker discovery. Further analysis of the biochemical pathways of significant proteins should improve our understanding of CDK