Echinococcus granulosus glycoconjugates induce peritoneal B cell differentiation into antibody secreting cells and cytokine production.

MOURGLIA-ETTLIN G,; AMEZCUA VESELY, MARIA CAROLINA; FRAGA R; BAZ, A; MERINO MC; GRUPPI A; DEMATTEIS SILVIA

WILEY-BLACKWELL PUBLISHING, INC

Año: 2011 p. 621 - 621

Helminth parasite infections are associated with predominant Th2-type cytokine responses, and parasite glycoconjugates have been recognized as partially responsible for such immune bias. It has been proved that Echinococcus granulosus evokes a Th2-type cytokine pattern characterized by a high production of IL-4, IL-5, IL-6 and IL-10, and no or mild IFN-γ levels in animal models and in patients with cystic echinococcosis, respectively. Here, we show that E4(+) (a glycoconjugate-enriched fraction from E. granulosus protoscolex) stimulated the secretion of a high concentration of IL-6, followed by IL-10 and TNF-α by normal peritoneal B cells. We determined that E4(+) bound to the surface of peritoneal B cells and induced their activation and, also, triggered the differentiation of peritoneal B cells into IgM-, IgG2b- and IgG3-secreting cells in a T-independent way. Interestingly, the IgM released by E4(+) -stimulated peritoneal B cells from norm