Antibodies to T.cruzi cytosol acidic antigens (FIV) in Chagas´ disease recognize parasite cell surface and human heart epitopes.

GEA, SUSANA; GRUPPI ADRIANA; BASSO, BEATRIZ; MENSO, EMILIA; VOTTERO-CIMA, E

JOHN WILEY & SONS INC

Año: 1990 p. 183 - 183

ntibodies to T.cruzi cytosol acidic antigens (FIV) in Chagas´ disease recognize parasite cell surface and human heart epitopes. S. Gea, A. Gruppi, B. Basso. E. Menso and E. Vottero-Cima. Journal of Clinical and Laboratory Immunology 131: 183-187, 1990. ISSN 0141-2760. Teviot-Kimpton Publications. Edinburgh (United Kindom). The expression of T. cruzi electronegative antigens (FIV) on the parasite surface and their cross-reactivity with heart tissue antigens was studied. For the former purpose epimastigotes (EPI) treated with glutaraldehyde were used to absorb antibodies against surface antigens. Glutaraldehyde fixed heart tissue was used for absorption of antibodies in sera from two groups of chagasic patients with normal and altered electrocardiogram. The absorption of sera from normal electrocardiogram group with EPI significantly reduced the anti FIV activity by ELISA (p less than 0.001). The decreased reactivity was observed with the antigenic bands focused at pI about 4.5.