CD8 + T Cell Immunity Is Compromised by Anti-CD20 Treatment and Rescued by Interleukin-17A

FIOCCA VERNENGO, FACUNDO; BECCARIA, CRISTIAN G.; ARAUJO FURLAN, CINTIA L.; TOSELLO BOARI, JIMENA; ALMADA, LAURA; GOROSITO SERRÁN, MELISA; GAZZONI, YAMILA; MONTES, CAROLINA L.; ACOSTA RODRÍGUEZ, EVA V.; GRUPPI, ADRIANA

mBio

ASM

Año: 2020 vol. 11

BSTRACT Treatment with anti-CD20, used in many diseases in which B cells play a pathogenic role, has been associated with susceptibility to intracellular infections. Here, we studied the effect of anti-CD20 injection on CD8􏰁 T cell immunity using an experimental model of Trypanosoma cruzi infection, in which CD8􏰁 T cells play a pivotal role. C57BL/6 mice were treated with anti-CD20 for B cell depletion prior to T. cruzi infection. Infected anti-CD20-treated mice exhibited a CD8􏰁 T cell response with a conserved expansion phase followed by an early contraction, resulting in a strong reduction in total and parasite-specific CD8􏰁 T cell numbers at 20days postinfection. Anti-CD20 injection increased the frequency of apoptotic CD8􏰁 T cells, decreased the number of effector and memory CD8􏰁 T cells, and reduced the fre- quency of proliferating and cytokine-producing CD8􏰁 T cells. Accordingly, infected anti-CD20-treated mice prese