Phytotoxic Potential of Secondary Matabolites of Fluorensia Species

ARJONA MILA R; A. DEL V. PACCIARONI; ARIZA ESPINAR , L.; SOSA V. E.

INST HISTOL EMBRIOL-CONICET

Lugar: Mendoza; Año: 2010 vol. 34 p. 19 - 19

his aim of this work was the isolation and identification of secondary metabolites in the hexane fraction ofFlourensia blakeana Dillon and the evaluation of the possible phytotoxic activity of the isolated metabolites. Vegetable material -flowers and leaves- was processed using the classical drying, grinding and ethanol-extraction method. Raw extract was re-suspended in MeOH-H2O 20% and partitioned with hexane, methylene chloride (Cl2CH2) and ethyl acetate (EtAcO). In the hexane fraction two compounds were isolated and identified as sesquiterpenes whose structures were elucidated through diverse UV, IR, RMN 1 H and 13 C spectroscopic methods. Activity assays were performed with concentrations of 10, 75 and 150 mg/L of the pure compounds. Lactuca sativa and Sorghum saccharatum seeds were used. Germination inhibition and root growth were determined. Data were analysed using ANOVA tests for a randomized design with three repetitions. Isolated sesquiterpenes produce inhibitory effects on