Antifungal photosensitive activity of Porophyllum obscurum (Spreng.) DC.: Correlation of the chemical composition of the hexane extract with the bioactivity

POSTIGO, AGUSTINA; FUNES, MATÍAS; PETENATTI, ELISA; BOTTAI, HEBE; PACCIARONI, ADRIANA; SORTINO, MAXIMILIANO

Photodiagnosis Photodynamic Therapy

ELSEVIER SCIENCE BV

Año: 2017 vol. 20 p. 263 - 263

1572-1000

e report Porophyllum obscurum as a source of new photosensitizers with potential use in Photodynamic Therapy as an alternative for oropharyngeal candidiasis treatment. The antifungal photosensitive activity of different extracts from P. obscurum was evaluated by using microdilution and bioautographic assays. The Minimum Fungicidal Concentration for hexanic extract under UV-A irradiation was 0.98 μg/mL, but it was inactive in experiments without irradiation. The bioassay-guided fractionation of this extract led to the isolation of four thiophenes responsible for the photosensitive activity: 2,2′:5′2″terthiophene, 5-(3-buten-1-ynyl)-2,2′-bithiophene, 5-(4-acetoxy-1-butenyl)-2,2′- bithiophene and 5-(4-hydroxy-1-butenyl)-2,2′- bithiophene, with Minimum Fungicidal Concentrations ranging 0.24-7.81 μg/mL under UV-A irradiation. The activity of the hexanic extract was evaluated against 25 clinical strains of Candida spp. isolates as etiological ag