Resumen:
ew Fuchsin (NF), also known as Magenta III, has potential
applications in photodynamic therapy. The commercial product
labeled NF contains two other dye components in different
proportions, Magenta II and Magenta I (Rosaniline).
The proportions of NF, Magenta II, and Magenta I determined by
reversed-phase high-performance liquid chromatography (RPHPLC)
in the commercial sample used were 71.6±0.4%, 25.2 ±
0.2%, and 2.8 ± 0.1% (n = 7), respectively. The isolation,
purification, and characterization of commercial NF dye
components were carried out applying different techniques, such as
preparative column liquid chromatography (PCLC), thin layer
chromatography (TLC), RP-HPLC, absorption spectrophotometry,
nuclear magnetic resonance spectroscopy (NMR), electrospray
ionization mass spectrometry (ESI-MS), and tandem electrospray
ionization mass spectrometry (ESI-MS–MS). After separation and
isolation, the degree of purity obtained for NF compound was
higher than 95% and 92% f