Simultaneous Determination of Bifonazole and Tinctures of Calendula Flower in Pharmaceutical Creams by Reversed-Phase Liquid Chromatography

CAROLA F. FERREYRA Y CRISTINA S ORTIZ

AOAC INT

Año: 2005 p. 1 - 1

he aim of this research was to develop and validate a sensitive, rapid, reproducible, easy and precise reverse-phase high-performance liquid chromatographic method for the stability studies of Bifonazole (I) formulated with Tinctures of Calendula Flower (II). The method was especially developed for the analysis and quantitative determination of I and II in pure and combinate forms in cream pharmaceutical formulations without using gradient elution and at room temperature. The influence on the stability of compound I as regards temperature, artificial radiation and compound II used for the new pharmaceutical design was evaluated. The LC separation was carried out using a Supelcosil LC-18 column (25 cm x 4.6 mm id, 5