Qualitative and Quantitative Reversed-Phase Liquid Chromatography of a New Bis-Isoxazolylnaphthoquinone

CAROLA F. FERREYRA, CRISTINA S ORTIZ Y MARÍA M. DE BERTORELLO

AOAC INT

Año: 2002 p. 349 - 349

he main objective of this study was to develop and test the applicability of a sensitive, accurate and precise high performance liquid chromatographic (HPLC) method for evaluating stability characteristics of a new bis-isoxazolylnaphthoquinone, the 2-(3,5-dimethyl-4-isoxazolylamino)-N-(3,5-dimethyl-4-isoxazolyl)-1,4-naphthoquinone-4-imine The assays showed to be selective and stability indicating. Isocratic elution with a mobile phase containing methanol-water, 75:25 (v/v), on a reverse-phase column with UV detection at ambient temperature completely resolved 1 from its degradation products. The HPLC system was calibrated by plotting peak responses versus known concentrations of a reference standard, using an internal standardization procedure. Complete elution occurred after 12 min with a peak symmetry factor of 0.95 for the drug peak. The kinetic degradation of was studied over the pH range from 0.88 to 14.00  in order to