Photodynamic properties andphotoinactivation of Candida albicansmediated by brominated derivatives oftriarylmethane and phenothiazinium dyes

M. GABRIELA ALVAREZ, M. NOEL MONTES DE OCA, M. ELISA MILANESIO, CRISTINA S. ORTIZ, EDGARDO N. DURANTINI

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2014 vol. 11 p. 148 - 148

he photodynamic activity of brominated derivatives of New Fuchsin and AzureB was studied in solution and in cell suspensions of Candida albicans. The spectroscopic andphotodynamic properties of these photosensitizers were compared with those of Crystal Vio-let and Azure B, which represent active photosensitizer related to each family of compounds.Triarylmethane derivatives absorb intensely with a band centered at ∼570 nm, while the phe-nothiazinium dyes at ∼650 nm. Photooxidation of 9,10-dimethylanthracene was observed usingphenothiazinium compounds indicating the formation of singlet molecular oxygen, while it wasnot detected using triarylmethane agents. However, triarylmethane dyes were able to photoox-idize L-tryptophan. In yeast cell suspensions, the photosensitized inactivation of C. albicansincreases with photosensitizer concentration, causing a ∼5 log decrease of cell survival, whenthe cultures are treated with 20 M of Crystal Violet and irradiated for 60 min.