Study of the destabilizing effect of perfluoro decanoic acid on models membranes.

BENJAMIN N. VIADA; CANDELARIA I. CAMARA ; ANA V. JUAREZ ; LIDIA M. YUDI

Buenos Aires

Congreso; I Reunión Conjunta de Sociedades de Biociencias; 2017

Sociedades de Biociencias

Perfluorinated acids could have harmful effects on living organisms, a possible hypothesis states that they may interact with biological membranes destabilizing them. Due to the difficulty of carrying out these in vivo studies, a more simplified model of artificial membrane was proposed, consisting of phospholipids adsorbed at water / air interfaces. In this case, distearoyl phosphatidic acid (DSPA), dilauryl phosphatidic acid (DLPA) and distearoyl phosphatidyl ethanolamine (DSPE) monolayers were used as the membrane model and their interaction with perfluro decanoic acid was studied (PFD). Langmuir isotherms (surface pressure, π, as a function of area per molecule) were performed and the permeability of the perfluorinated acid on said monolayers was evaluated. The obtained isotherms demonstrate that PFD penetrates all the studied monolayers by expanding them. Brewster Angle Microscopy (BAM) shows that PFD affects the formation of lipid domains by destabilizing them. Finally, the detergent power of PFD on DLPA and DSPA large unilamellar vesicles (LUVs) was determined.