Molecular mechanisms implicated in galectin-1-induced

RABINOVICH GA; ALONSO CR; SOTOMAYOR CE; DURAND S; BOCCO JL; RIERA CM

NATURE PUBLISHING GROUP

Año: 2000 vol. 7 p. 747 - 747

font size="2" face="AdvP6EC5"> Galectins are emerging as a new class of bioactivemolecules with specific immunomodulatory properties. Galectin-1 (Gal- 1), a member of this family, has been shown to induce apoptosis of mature T cells and immature thymocytes. To gain insight into the intracellular signals transduced by Gal-1 upon binding to mature T cells, we investigated whether this protein triggered activation of the dimeric AP-1 transcription factor. A marked increase in the binding of nuclear extracts to synthetic oligonucleotides containing the AP-1 consensus sequence, could be detected by an electrophoretic mobility shift assay, when T cells were cultured for 30 min in the presence of Gal-1. This DNA-bindi