Protective phenotypes of club cells and alveolar macrophages are favored as part of endotoxin-mediated prevention of asthma

GARCÍA LN; LEIMGRUBER C; URIBE ECHEVARRÍA EM; ACOSTA PL; BRAHAMIAN JM,; POLACK FP; MIRÓ MS,; QUINTAR A; SOTOMAYOR CE; MALDONADO C

SOC EXPERIMENTAL BIOLOGY MEDICINE

Lugar: Maywood; Año: 2014 p. 1 - 1

topic asthma is a chronic allergic disease that involves T-helper type 2 (Th2)-inflammation and airway remodeling. Bronchiolar club cells (CC) and alveolar macrophages (AM) are sentinel cells of airway barrier against inhaled injuries, where allergy induces mucous metaplasia of CC and the alternative activation of AM, which compromise host defense mechanisms and amplify Th2- inflammation. As there is evidence that high levels of environmental endotoxin modulates asthma, the goal of this study was to evaluate if the activation of local host defenses by Lipopolysaccharide (LPS) previous to allergy development can contribute to preserving CC and AM protective phenotypes. Endotoxin stimulus before allergen exposition reduced hallmarks of allergic inflammation including eosinophil influx, Interleukin-4 and airway hyperreactivity, while the T-helper type 1 related cytokines IL-12 and Interferon-g were enhanced. This response was accompanied by the preservation of the normal CC phenot