Role of ACDI, a putative DNA glycosylase, in the Arabidopsis thaliana development

NOTA F; CAMBIAGNO D; ALVAREZ MARIA ELENA

Puerto Madryn

Congreso; XLVI Reunión SAIB; 2010

SAIB

The Arabidopsis genome suffers a massive DNA demethylation and centromeric chromatin deconsensation in response to Pseudomonas attack. We speculate that the first response involves the action of DNA glycosylases which mediate demethylation of cytosine residues acting together with Base Excition Repair system. In Arabidopsis, the DNA glycosylases belonging to the DEMETER (DME) family are the best characterized. This family includes DEMETER, ROS1, DEMETER-LIKE 2 and DEMETER_LIKE 3. We are studying a novel putative DNA glycosylase, named ACDI, which carries an Helix-harpin-Helix DNA glycosylase Domain, and differs from the DEMETER family members in its secondary structure with. We have previously reported that ACDI affects chromatin decondensation during infection with Pseudomonas. Here we evaluate the involvement of ACDI in vegetative and reproductive development of Arabidopsis, and analyze the transcript level of ACDI in DME family plant mutants. Our results suggest that ACDI may be involved in regulation of developmental processes. Moreover, we found an enhanced level of ACDI transcript in some DME family plant mutants suggesting some compensation effect between these transcripts.