Effectiveness of ZnPc and of an amine derivative to inactivate Glioblastoma cells by Photodynamic Therapy: an in vitro comparative study

PRUCCA, CG

Scientific Reports

Nature Publishing Group

Año: 2019

2045-2322

lioblastoma multiforme is considered to be one of the most aggressive typesof tumors of the central nervous system, with a poor prognosis and shortsurvival periods of ~ one year. The current protocol for glioblastomatreatment includes the surgical excision of the primary tumor followed byradio and chemotherapy. Photodynamic therapy (PDT) is considered apromising strategy for the treatment of several types of tumors.Phthalocyanines (Pcs) are good photosensitizers (PSs) for PDT because theyinduce cell death in several cellular models. ZnPc (Zn(II)phthalocyanine) is awell-known Pc, extensively tested in different cells and tumor models, but itsevaluation on a glioblastoma model has been poorly studied. Herein, wecompare the capacity of ZnPc and one of its derivatives,Zn(II)tetraminephthalocyanine (TAZnPc), to photoinactivate glioblastomacells (T98G, MO59, LN229 and U87-MG) in culture. We measured thecellular uptake, the toxicity in the dark and the subcellular localization of thedifferen