Expansion of myeloid-derived suppressor cells with arginase activity lasts longer in aged than in young mice after CpG-ODN+IFA treatment.

MARÍA F. HARMAN, ROMINA P. RANOCCHIA, CAROLINA V. GORLINO, MARÍA F. SÁNCHEZ VALLECILLO, SOFÍA D. CASTELL, MARÍA I. CRESPO, BELKYS A. MALETTO, GABRIEL MORÓN AND MARÍA C. PISTORESI-PALENCIA

oncotarget

Oncotarget

Año: 2015

1949-2553

s we age, the homeostatic function of many systems in the body, such as the immune function declines, which in turn contributes to augment susceptibility to disease. Here we describe that challenging aged mice with synthetic oligodeoxynucleotides containing unmethylated cytosine guanine motifs (CpG-ODN) emulsified in incomplete Freund´s adjuvant (IFA), (CpG-ODN+IFA) an inflammatory stimulus, led to the expansion of CD11b+Gr1+ myeloid cells with augmented expression of CD124 and CD31. These myeloid cells lasted longer in the spleen of aged mice than in their younger counterparts after CpG-ODN+IFA treatmen and were capable of suppressing T cell proliferative response by arginase induction. Myeloid cells from aged CpG-ODN+IFA-treated mice presented increased arginase-1 expression and enzyme activity. In addition, we found a different requirement of cytokines for arginase induction according to mice age. In myeloid cells from young treated mice, arginase-1 expression and activity is induc