A liquid crystal of ascorbyl palmitate, used as vaccine platform, provides sustained release of antigen and has intrinsic pro-inflammatory and adjuvant activities which are dependent on MyD88 adaptor protein

SÁNCHEZ VALLECILLO MF, MINGUITO DE LA ESCALERA MM, AGUIRRE MV, ULLIO GAMBOA GV, PALMA SD, GONZÁLEZ-CINTADO L, CHIODETTI AL, SOLDANO G, MORÓN G, ALLEMANDI DA, ARDAVÍN C, PISTORESI-PALENCIA MC, MALETTO BA

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2015 p. 12 - 12

odern subunit vaccines require the development of new adjuvant strategies. Recently, we showed that CpG-ODN formulated with a liquid crystal nanostructure formed by self-assembly of 6-O-ascorbyl palmitate (Coa-ASC16) is an attractive system for promoting an antigen-specific immune response to weak antigens. Here, we showed that after subcutaneous injection of mice with near-infrared fluorescent dye-labeled OVA antigen formulated with Coa-ASC16, the dye-OVA was retained at the injection site for a longer period than when soluble dye-OVA was administered. Coa-ASC16 alone elicited a local inflammation, but how this material triggers this response has not been described yet. Although it is known that some materials used as a platform are not immunologically inert, very few studies have directly focused on this topic. In this study, we explored the underlying mechanisms concerning the interaction between Coa-ASC16 and the immune system and we found that the whole inflammatory response elic