Orally Administered OVA/CpG-ODN Induces Specific Mucosal and Systemic Immune Response in Young and Aged Mice

D. ALIGNANI, B. MALETTO, M. LISCOVSKY, A. RÓPOLO, G. MORÓN, M. C. PISTORESI-PALENCIA.

JOURNAL OF LEUKOCYTE BIOLOGY

The Society for Leukocyte Biology

Año: 2005 vol. 77 p. 898 - 898

0741-5400

e have previously demonstrated that subcutaneously administered ovalbumin (OVA) plus synthetic oligodeoxynucleotides containing immunostimulatory CpG motifs (CpG-ODN) as adjuvant stimulate cellular and humoral immunity and promote T helper cell type 1 differentiation in aged mice. The present study assessed the ability of CpG-ODN to induce an OVA-specific immune response after oral immunization in young (3-month-old) and aged (18-month-old) BALB/c mice. Oral OVA/CpG-ODN immunization induces a similar OVA-specific T cell-proliferative response (in mucosal and systemic tissues), immunoglobulin G (IgG) in plasma, and IgA in intestinal washes in both groups of ages. The OVA-specific humoral immune response observed in aged mice was similar to the one observed in young mice, peaking at day 7 after the last oral immunization and was present over 40 days after the last oral immunization. The pattern of cytokines released in culture supernatants in both groups of mice was similar, with specif