A natural oil increases specific anti-OVA IgG levels and induces a cellular immune response combinedwith aluminum hydroxide

NOELIA CAMPRA; IVANA MONTIRONI; ELINA REINOSO; JOSE RAVIOLO; FEDERICO RUIZ MORENO; MALETTO, BELKYS ANGÉLICA; LAURA CARIDDI

JOURNAL OF LEUKOCYTE BIOLOGY

FEDERATION AMER SOC EXP BIOL

Lugar: Bethesda; Año: 2021

0741-5400

he efficacy of certain vaccines is improved by the use of adjuvants. Nowadays, the developmentof new, effective, and safe adjuvants that stimulate the innate immune response is researched. In this context, medicinal plants appear as a suitable alternative. Minthostachys verticillata essential oil (EO) has demonstrated the ability to modulate mechanisms of the innate immune response.Thus, the present work aimed to evaluate the EO adjuvant effect on humoral and cellular immunity, coadministered with OVA as antigen. The chemical analysis of EO by gas chromatography-mass spectrometry revealed a predominant pulegone-menthone chemotype. EO (1.25, 2.5, or 5.0 mg/ml) did not alter the viability of murine fibroblasts (3T3 cell line) neither showed signs of toxicity in Balb/c mice inoculated subcutaneously. The serum of mice immunized with OVA + EO showed increased levels of anti-OVA-specific antibodies of IgG1 subclass compared with the mice immunized with OVA alone revealing an adjuvant effec