Dehydration of erythrocytes parasitized with Babesia spp as an immunoprophylactic alternative. Preliminary results Deshidratación de eritrocitos parasitados con Babesia spp como alternativa inmunoprofiláctica. Resultados preliminares

RÍO ÁLVAREZ, FLORENCIA DEL; PEICHOTO, MARÍA E.; PALMA, SANTIAGO; MALETTO, BELKYS; GUIDOLI, MARCOS; HUBER, LAURA; LOZINA, LAURA

Revista de Investigaciones Veterinarias del Peru

Universidad Nacional Mayor de San Marcos

Año: 2022 vol. 33

1682-3419

mmunoprophylaxis for bovine babesiosis has two presentations of a live attenuated vaccine (fresh and deep-frozen). Despite being effective, their durability, as well as the conditions necessary for their transfer and handling, make them impractical. The incorporation of a third presentation, using dehydrated merozoites as immunogens, constitutes an innovative alternative, which combines practicality and stability over time. The objective of this work was to test dehydration processes and cryopreservative and rehydrating substances. For this, erythrocytes highly parasitized with Babesia bovis and B. bigemina were subjected to two dehydration techniques: lyophilization and spray drying. In turn, for the lyophilization process, several lyoprotectants were tested: Dimethylsulfoxide, Glycerol, Dextrose and Polyvinylpyrrolidone (PVP). The only one that achieved a properly dehydrated powder, crystalline in appearance and brittle to the touch was the PVP. Subsequently, the erythrocytes obtain