VACCINE PLATFORM BASED ON THE USE OF ASCORBYL PALMITATE RESIST DIFFERENT STORAGE CONDITIONS

RUIZ MORENO, FEDERICO; CONSTANZA MARIN; GIULIANA VEDELAGO; DANIEL ALBERTO ALLEMANDI; SANTIAGO PALMA; MARIA CRISTINA PISTORESI PALENCIA; GABRIEL MORON; BELKYS MALETTO

Congreso; LXX REUNIÓN SAI; 2022

We have previously demonstrated that formulation of OVA and CpG-ODN in a nanostructure formed by self-assembly of 6-O-ascorbyl palmitate (Coa-ASC16) elicited antigen-specific antibody and cellular responses superior to those induced by the soluble counterpart. Here, we study the stability of the nanoformulation OVA/CpG-ODN/Coa-ASC16 stored for 2 days at room temperature and for 30 days at 4°C, using a newly prepared nanoformulation as a control. Mice were subcutaneously immunized with a single dose of OVA and CpG-ODN nanoformulated with Coa-ASC16 (OVA/CpG-ODN/Coa-ASC16) maintained under the different storage conditions. Then, we evaluated by flow cytometry the SIINFEKL-Kb tetramer+ CD8+ T cells and INFɣ produced by spleen cells upon in vitro rechallenge with OVA as well as OVA-specific antibody response by ELISA, OVA-specific antibody and cellular responses elicited were similar among the three groups of mice regardless of the formulation used (p:ns). These data showed that the ambient temperature as well as the conservation short time of the nanoformulation does not produce changes on immune response elicited, an indicator of its biological function.