RAMAN SPECTROSCOPY UNCOVERS BIOLOGICAL CHANGES IN EXTRACELLULAR VESICLES GENERATED BY THYROID TUMOR CELL-FIBROBLAST INTERPLAY

MÓNICA BEATRIZ GILARDONI

MEDICINA (BUENOS AIRES)

MEDICINA (BUENOS AIRES)

Lugar: Buenos Aires; Año: 2022 vol. 82

0025-7680

xtracellular vesicles (EVs) are a heterogeneous population of nanoparticles involved in cell-cell communication. Carcinoma is a complex society of interacting cells, with tumor-stroma crosstalk leading to tumor progression.We demonstrated that thyroid tumor cell-fibroblast (Fb) interaction induces the secretion of metalloproteinases (MMPs) to culture supernatants (CMs) and promotes a migratory phenotype in the tumor cells. Thyroid tumor cell-Fb crosstalk liberates EVs that are involved in extracellular matrix remodelling. Raman spectroscopy is a well-established label-free and non-destructive method for determining chemical characteristics in biological samples. We utilized Raman Confocal Microspectroscopy (Raman-CM) to explore individual secreted-EVs and to describe the impact of tumor-stroma interplay in their chemical make-up. Thyroid tumor cells (TPC-1) or thyroid non-tumor cells (NThyOri) were co-cultured with normal human Fb as a simulation of the thyroid tumor microenvironment