Thyroid hormones-membrane interaction: Reversible association of hormones with organized phospholipids with changes in fluidity and dipole potential.

ISSE, BA., ; YUNES QUARTINO PJ.,; FIDELIO GD.,; FARIAS RN

ELSEVIER IRELAND LTD

Lugar: Amsterdam; Año: 2013 vol. 175 p. 131 - 131

h2 class="secHeading" id="section_abstract">Abstract Differential scanning calorimetry (DSC), mixed monomolecular layers and fluorescence spectroscopy techniques were applied to investigate the effect of thyroid hormones (THs) on the biophysical properties of model membranes. We found that both 3,3¡ä,5-triiodo-l-thyronine (T3) and 3,5,3¡ä,5¡ä-tetraiodo-l-thyronine (T4) induce a broadening of the calorimetric main phase transition profile and reduce the transition enthalpy in liquid-crystalline state of dipalmitoylphosphatylcholine (DPPC) multilamellar vesicles. Tm changes from 41 ¡ãC to 40 ¡ãC compared to pure DPPC. When the experiments were done by adding THs to preformed multilamellar vesicles a second broader component in the DSC scan also appears at 20 min of incubation and becomes gradually more prominent with time, indicating a progressive altera